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An amber mutation in the gene rpsA for ribosomal protein S1 in Escherichia coli

✍ Scribed by Kitakawa, Madoka ;Isono, Katsumi

Publisher: Springer
Year: 1982
Tongue: English
Weight: 361 KB
Volume: 185
Category: Article
ISSN: 0026-8925
DOI: 10.1007/bf00334137

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✦ Synopsis

An amber mutation has been induced in the gene rpsA (which codes for ribosomal protein S1) of Escherichia coli K-12 strain in the presence of an amber suppressor (supD) and mutations sueA, sueB and sueC that additively enhance the efficiency of suppression. That the amber mutation has occurred in the gene rpsA was confirmed by complementation with a plasmid which carried the wild-type allele of rpsA. The mutation is lethal in the absence of an amber suppressor, indicating that ribosomal protein S1 is indispensable to E. coli.

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