Comparative studies on nucleoside diphosphatase of rat ascites hepatoma and rat liver: Activity level, purification and properties

Studies on the mechanisms of invasion in

Studies on the mechanisms of invasion in cancer. III. Purification of a neutral protease of rat ascites hepatoma cell associated with production of chemotactic factor for cancer cells

✍ Masashi Koono; Kenichiro Ushijima; Hideo Hayashi 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 French ⚖ 806 KB

## Abstract A neutral protease was extracted from rat ascites hepatoma cells and effectively purified by fractionation with ammonium sulfate followed by chromatography using TEAE‐cellulose and hydroxylapatite in that order. The protease eluted in 120 mM phosphate buffer (pH 6.8) on hydroxylapatite,