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Comparative studies of hemoglobins from the clouded tiger salamander before and after metamorphosis

✍ Scribed by Woody, A-Young ;Justus, J. T.

Publisher: John Wiley and Sons
Year: 1974
Tongue: English
Weight: 621 KB
Volume: 188
Category: Article
ISSN: 0022-104X
DOI: 10.1002/jez.1401880209

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✦ Synopsis

Abstract

Some comparative biochemical studies on the hemoglobin from the clouded tiger salamander, Ambystoma tigrinum nebulosum, before and after metamorphosis indicate (1) there is no hemoglobin change with metamorphosis; (2) both hemoglobins show three major fractions (Fractions I, II, III) in ion exchange chromatography and two bands in gel electrophoresis; (3) these fractions possess identical chemical properties except for their electrophoretic mobilities, and peptide maps; (4) Under our experimental conditions, Fraction II can be formed by the appropriate recombination of subunits present in equilibrium with Fractions I and II.

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