Comparative genomic hybridization as a tool to define two distinct chromosome 12-derived amplification units in well-differentiated liposarcomas

✍ Scribed by Ron F. Suijkerbuijk; Daniel E. M. Olde Weghuis; Margot Van Den Berg; Ad Geurts Van Kessel; Florence Pedeutour; Claude Turc-Carel; Anne Forus; Ola Myklebost; Cliff Glier

• Book ID: 102221896
• Publisher: John Wiley and Sons
• Year: 1994
• Tongue: English
• Weight: 379 KB
• Volume: 9
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870090410

✦ Synopsis

Well-differentiated liposarcomas (WDLPS) are frequently characterized by a near-diploid karyotype with supernumerary ring andlor giant rod-shaped marker chromosomes. We have shown, using fluorescence in situ hybridization (FISH) and molecular strategies, that these markers contain chromosome 12-derived sequences. Here we report the analysis of six WDLPS for the presence of amplified D N A segments by means of the recently developed comparative genomic hybridization (CGH) strategy. Two distinct chromosome 12-derived amplification units could be identified in all tumors examined, one located in the q 14-q I 5 region as expected, the second unexpectedly mapping to q2 I .3-q22. Our results indicate that the concerted amplification of these two distinct regions on the long arm of chromosome I 2 may be a consistent characteristic of WDLPS. These amplifications are most likely directly related to the presence of supernumerary ring and/or giant marker chromosomes in this group of soft tissue tumors. Genes Chrom Cancer 9292-295 (1994).