Comparative genomic hybridization analysis of human sarcomas: I. Occurrence of genomic imbalances and identification of a novel major amplicon at 1q21–q22 in soft tissue sarcomas

✍ Scribed by Anne Forus; Daniël Olde Weghuis; Dominique Smeets; Øystein Fodstad; Ola Myklebost; Ad Geurts van Kessel

• Publisher: John Wiley and Sons
• Year: 1995
• Tongue: English
• Weight: 624 KB
• Volume: 14
• Category: Article
• ISSN: 1045-2257
• DOI: 10.1002/gcc.2870140103

✦ Synopsis

Comparative genomic hybridization (CGH) was recently developed as a tool to survey entire genomes for variations in DNA sequence copy numbers. We have applied this technique to detect and map amplified regions in 54 soft tissue sarcomas.

Aberrations were detected by visual analysis of hybridizations or contrast-enhanced digital images, followed by quantitative digital ratio imaging of the aberrant chromosomes. Several tumors showed increased D N A sequence copy number at 12q 14, as expected. However, CGH analysis detected amplification of 12q 14 also in some tumors where neither MDMZ nor C D M was amplified, suggesting that another as yet unknown gene(s) may drive amplification of this region in sarcomas. Furthermore, a novel recurring arnplicon was detected at I q2 I -q22. D N A amplifications coinciding with this segment were as frequent as those observed for 12q14, indicating that Iq21q22-linked gene(s) may also play an important role in the development and/or progression of human soft tissue sarcomas. Genes Chrornosorn Cancer 14:8-14 (1995).