Comparative analysis of different TaqMan real-time RT-PCR assays for the detection of swine Hepatitis E virus and integration of Feline calicivirus as internal control

Validation of single real-time TaqMan® P

Validation of single real-time TaqMan® PCR assay for the detection and quantitation of four major genotypes of hepatitis E virus in clinical specimens

✍ V. Enouf; G. Dos Reis; J.P. Guthmann; P.J. Guerin; M. Caron; V. Marechal; E. Nic 📂 Article 📅 2006 🏛 John Wiley and Sons 🌐 English ⚖ 205 KB 👁 1 views

Since the characterization of the genome of the hepatitis E virus (HEV) in 1990, a large genetic diversity has been described. A single real-time reverse transcription (RT)-PCR assay with TaqMan technology has been validated which uses only one set of primers and probe within the ORF2 HEV region (nt