Comparative 1H NMR studies of saturation transfer in copolymer gels and mouse lenses

✍ Scribed by Koji Nakamura; Masaru Sogami; Seiichi Era; Shigeru Matsushima; Yasutomi Kinosada

• Publisher: John Wiley and Sons
• Year: 2010
• Tongue: English
• Weight: 182 KB
• Volume: 23
• Category: Article
• ISSN: 0952-3480
• DOI: 10.1002/nbm.1499

✦ Synopsis

Abstract

Saturation transfer in cross‐linked copolymer gels and excised intact and perforating trauma‐induced cataract mouse lenses (4‐ or 8‐week‐old) were studied using intermolecular cross‐relaxation rates (1/T~IS~(H~2~O); 1/T~IS~), monitored with f~2~‐irradiation at −8.79, −4.00, and 7.13 ppm (γH~2~/2__π__ ∼ 69 Hz). [1] The 1/T~IS~(7.13 ppm) vs dry weight [W (%)] profiles for hydrophilic copolymer gels were far steeper than those for hydrophobic copolymer gels, indicating the participation of an amount of bound water and a number of copolymer hydroxyl groups in the saturation transfer process. In contrast, the 1/T~IS~(−8.79 ppm) vs W (%) profiles exhibited little difference between the hydrophilic and hydrophobic copolymer gels, indicating the major participation of molecular rigidity, i.e. W (%) in the saturation transfer process. [2] The 1/T~IS~(7.13 ppm) values for cataractous mouse lenses were larger than those for intact lenses, indicating the formation of large, immobile lens protein associates or aggregates containing a sufficient amount of bound water for the saturation transfer. [3] The 1/T~IS~(7.13 ppm) vs W (%) profiles for the hydrophilic copolymer gels exhibited similar characteristics to the intact and cataractous mouse lenses with regard to the saturation transfer process. Copyright © 2010 John Wiley & Sons, Ltd.