✦ LIBER ✦

Diffusion of solutes in agarose and alginate gels: 1H and 23Na PFGSE and 23Na TQF NMR studies

✍ Scribed by Peter Lundberg; Philip W Kuchel

Publisher: John Wiley and Sons
Year: 1997
Tongue: English
Weight: 955 KB
Volume: 37
Category: Article
ISSN: 0740-3194
DOI: 10.1002/mrm.1910370108

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Cells immobilized in gels experience potential metabolic restrictions in the form of reduced diffusion rates of metabolites and ions and their possible selective adsorption on the gel matrix. Diffusion and relaxation characteristics of common solutes in agarose and barium alginate gels were investigated at 37°C by using ^1^H PFGSE and ^23^Na TQF NMR spectroscopy. Glucose, glycine, alanine, lactate, sodium ions, and HDO were studied. There were no selective interactions between any of the metabolites and the gel materials but the diffusion coefficients were uniformly reduced. The effects of metabolite diffusion and utilization, in gel beads and threads containing cells, were simulated by using a reaction diffusion model incorporating the measured diffusion coefficients. Metabolism is expected to be very significantly limited by diffusion of solutes to and from the cells that are centrally located within gel threads or spheres of radius ∼2.0 mm, which is a cornmonly used size.

📜 SIMILAR VOLUMES

31P NMR and triple quantum filtered 23Na

31P NMR and triple quantum filtered 23Na NMR studies of the effects of inhibition of Na+/H+ exchange on intracellular sodium and pH in working and ischemic hearts

✍ Gil Navon; Jeffrey G. Werrmann; Ron Maron; Sheila M. Cohen 📂 Article 📅 1994 🏛 John Wiley and Sons 🌐 English ⚖ 920 KB

## Abstract The triple quantum filtered ^23^Na NMR method is applied here to measure the effects of EIPA, a specific inhibitor of the Na^+^/H^+^antiporter, on relative intracellular sodium concentrations in isolated working hearts at baseline, during ischemia, and at subsequent reperfusion. In anal

An NMR study of 1H, 31P, and 23Na relaxa

An NMR study of 1H, 31P, and 23Na relaxation and molecular dynamics in the polycrystalline sodium salt of adenosine monophosphate

✍ E.C Reynhardt; S Froneman; E.R Andrew; E Szczesniak 📂 Article 📅 1989 🏛 Elsevier Science ⚖ 633 KB

Androgen sensitivity of rat prostate car

Androgen sensitivity of rat prostate carcinoma studied by 31P NMR spectroscopy, 1H MR imaging, and 23Na MR imaging

✍ D. B. Vigneron; H. Hricak; T. L. James; P. B. Jajodia; L. Nunes; P. Narayans 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 531 KB

3'P NMR spectroscopy, 'H magnetic resonance (MR) imaging, and 23Na MR imaging were used to study the biochemical difference between nine hormone-sensitive and six hormone-resistant rat prostate cancers and to follow bioenergetic and morphologic changes subsequent to androgen deprivation in the hormo

Lithium-7 and sodium-23 nmr studies of c

Lithium-7 and sodium-23 nmr studies of complexation of Li+ and Na+ ions with 1,10-phenanthroline, 2,2′-bipyridine and 8-hydroxyquinoline in some non-aqueous solutions

✍ Tayyebeh Madrakian; Abbas Afkhami; Jahanbakhsh Ghasemi; Mojtaba Shamsipur 📂 Article 📅 1996 🏛 Elsevier Science 🌐 English ⚖ 416 KB

ChemInform Abstract: Specific Features o

ChemInform Abstract: Specific Features of Synthesis of Fullerides MnC60 (M: Na, K, Rb) in Aromatic Hydrocarbons as Probed by 13C, 23Na, and 1H NMR.

✍ B. M. Bulychev; V. I. Privalov; A. A. Dityat'ev 📂 Article 📅 2000 🏛 John Wiley and Sons ⚖ 31 KB

1H and 23Na MRI studies of Atlantic salm

1H and 23Na MRI studies of Atlantic salmon (Salmo salar) and Atlantic cod (Gadus morhua) fillet pieces salted in different brine concentrations

✍ Emil Veliyulin; Ida G Aursand 📂 Article 📅 2007 🏛 John Wiley and Sons 🌐 English ⚖ 400 KB