Comments on cell adhesion to biomaterial surfaces: The origin of saturation in platelet deposition - is it kinetic or thermodynamic?

✍ Scribed by Ruckenstein, E. ;Srinivasan, R.

• Publisher: John Wiley and Sons
• Year: 1982
• Tongue: English
• Weight: 247 KB
• Volume: 16
• Category: Article
• ISSN: 0021-9304
• DOI: 10.1002/jbm.820160209

✦ Synopsis

A recent article by Neumann et al.,l deals with the deposition of pig platelets upon various collector surfaces. These authors performed the experiments for durations of up to 3 h and observed that the deposition saturated at a fractional coverage on each surface at about 11/2 h from the start. Neumann et al.,' proposed that the observed saturation is of the Langmuir type, implying that it is based on a thermodynamic equilibrium between deposition and the detachment of deposited cells. The authors have also concluded that an existing theory of Ruckenstein et a1.,* for cell deposition cannot explain these results. Contrary to these assertions, it is the intent of this Note to emphasize that saturation in cell deposition is of a kinetic origin and can be explained by adapting the theory of Ruckenstein et al.,2-5 to the present circumstances.

In cell deposition experiments such as the one cited above, deposition occurs from a stagnant suspension onto a flat horizontal collector. By gravity, the cells arrive at a distance of about 100 8, from the collector surface and in this range interact with the surface via van