𝔖 Bobbio Scriptorium
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Comment on R.A. White's ν function

✍ Scribed by Willi Schmidt

Publisher
John Wiley and Sons
Year
1996
Tongue
English
Weight
244 KB
Volume
24
Category
Article
ISSN
0196-4763

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✦ Synopsis

The v function has been defmed for the purpose of determining the potential doubling time (Tp,3 from bivariate flow cytometric data based on pulse labeling of the S-phase cells by bromodeoxyuridine (BrdUrd) or other related substances (White et a l . : Cytometry 11514-317,1990). One of the m e d i a t e applications of that method is the derivation of the S-phase duration by using relative movement (RM) analysis (Begg et al.: Cytometry 6:620-626, 1985).

With the v function the potential doubling time Tpf can be derived if exponential growth with stable age distribution and constant phase durations can be

The availability of monoclonal antibodies against bromodeoxyuridine (BrdUrd) has paved the way for monitoring the progression of cell cohorts which have incorporated BrdUrd in the DNA. Technically speaking, the kinetics of this progression can be studied by bivariate flow cytometry whereby DNA content and BrdUrd incorporation are measured simultaneously (2).

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