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Combined scanning optical coherence and two-photon-excited fluorescence microscopy

✍ Scribed by Beaurepaire, E.; Moreaux, L.; Amblard, F.; Mertz, J.

Book ID
115423632
Publisher
Optical Society of America
Year
1999
Tongue
English
Weight
837 KB
Volume
24
Category
Article
ISSN
0146-9592

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Simultaneous two-photon excited Β―uorescence (TPF) and second-harmonic generation (SHG) imaging is demonstrated using a single femtosecond laser and a scanning microscope. This composite nonlinear microscopic technique was applied to imaging DNA and chromosomes, and it was shown that the two differen