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Combinatorial protein design by in vitro recombination

✍ Scribed by Lori Giver; Frances H Arnold

Book ID
104415001
Publisher
Elsevier Science
Year
1998
Tongue
English
Weight
354 KB
Volume
2
Category
Article
ISSN
1367-5931

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✦ Synopsis

DNA recombination is a powerful engine for the creation of new phenotypes. Recently, methods for in vitro DNA recombination (DNA shuffling) have been developed and applied to the evolution of novel molecules in the laboratory. An exciting new development is the shuffling of homologous genes to create diversity for directed evolution.

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Illegitimate recombination dependent on T4 DNA topoisomerase in a cell-free system has recently been described. In that work, recombinants between two phage lambda DNA molecules were produced by the topoisomerase alone, without an Escherichia coli extract. In this paper, it is shown that recombinati