Column swithiching in high-pressure liquid chromatography
โ Scribed by J.F.K. Huber; R. Van Der Linden; E. Ecker; M. Oreans
- Book ID
- 104145026
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 731 KB
- Volume
- 83
- Category
- Article
- ISSN
- 1873-3778
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โฆ Synopsis
A high-pressure micro multiport switching valve, its performance and application are described. It is shown that the contribution of column switching to the peak width is negligible with this valve. Different modes of operation are demonstrated:
(I) Control of the total elution of the sample, by-passing the column.
(2) Stripping of a part of the sample, using a sectional column system.
(3) Separation of a complex mixture on a given phase system using columns of different phase ratio.
๐ SIMILAR VOLUMES
When high-speed liquid chromatography is used to obtain pure material for subsequent investigation, it can correctly be called preparative, regardless of the sample weights employed. In this paper, the requirements for scaling up from a smalldiameter analytical column to larger-diameter preparative