## Abstract Using a specially designed column system, we have systematically investigated the effect of mobile phase velocity on column efficiency. The performance of small bore columns operated at different linear velocities of mobile phase was examined for three different types of injection syste
Preparative columns in high-speed liquid chromatography
β Scribed by D.R. Baker; R.A. Henry Jr.; R.C. Williams; D.R. Hudson; N.A. Parris
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 733 KB
- Volume
- 83
- Category
- Article
- ISSN
- 1873-3778
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β¦ Synopsis
When high-speed liquid chromatography is used to obtain pure material for subsequent investigation, it can correctly be called preparative, regardless of the sample weights employed. In this paper, the requirements for scaling up from a smalldiameter analytical column to larger-diameter preparative columns are presented. An example of the use of high-speed preparative liquid chromatography to isolate and identify the components of a complex mixture is presented. General requirements of sample size for subsequent investigation are discussed.
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