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Column chromatography of amino acids with fluorescence detection

✍ Scribed by Marc Roth; Ayoub Hampaǐ

Publisher
Elsevier Science
Year
1973
Tongue
English
Weight
345 KB
Volume
83
Category
Article
ISSN
1873-3778

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✦ Synopsis

A highly sensitive fluorescence reaction for amino acids using o-phthalaldehyde and 2-mercaptoethanol permits the detection of amino acids separated on small ion-exchange columns. The advantages are: (I) good precision at the level of 0.5 nmole; (2) high speed due to the smaller height of the column; and (3) linear relationship between meter response and amino acid concentration.

The same reaction is also applicable to amino sugars.

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