Column liquid chromatography with diode laser-induced fluorescence detection of carboxylic acids after pre-column derivatisation

✍ Scribed by Arjan J.G. Mank; Marco C. Beekman; Nel H. Velthorst; Udo A.Th. Brinkman; Henk Lingeman; Cees Gooijer

• Publisher: Elsevier Science
• Year: 1995
• Tongue: English
• Weight: 737 KB
• Volume: 315
• Category: Article
• ISSN: 0003-2670
• DOI: 10.1016/0003-2670(95)00334-v

✦ Synopsis

Several pre-column approaches to fluorescence derivatisation of carboxylic acids, compatible with diode laser-induced fluorescence {DIO-LIF) detection, were compared. For this purpose red-absorbing labels containing hydrazide, bromoacetamide and iodoacetamide ftmctionalities were studied. The sensitivity of DIO-LIF detection is illustrated by the (3-10) X lo-" M (75-250 amol) detection limit for various derivatised carboxylic acids. The real-life detection limit for the carboxylic acids is 3 X lo-' M, because derivatisation is not quantitative at lower concentrations. Derivatised n-alkylcarboxylic acids and non-steroidal anti-inflammatory drugs are easily separated by column liquid chromatography, despite the attachment of the bulky red-absorbing labels. The dete~inarion of the anti-~~a~ato~ drug naproxen in saliva is shown as an example.