Colorimetric determination of ϵ-N-methyl-l-lysine
✍ Scribed by Woon Ki Paik; Sangduk Kim
- Publisher
- Elsevier Science
- Year
- 1965
- Tongue
- English
- Weight
- 400 KB
- Volume
- 13
- Category
- Article
- ISSN
- 0003-2697
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📜 SIMILAR VOLUMES
## Abstract The separation and resolution of the isopeptides __N__ϵ(γ‐glutamyl)‐L‐lysine and __N__ϵ(β‐aspartyl)‐L‐lysine formed in heated proteins has been successfully achieved. The method demands a well‐characterised ion‐exchange column and the use of pH 3.40 lithium citrate buffer (0.2 N Li^+^).
## Abstract The conformational changes of poly‐__N__^ε^‐glutaryl‐L‐lysine (PGL) and poly‐__N__^ε^‐succinyl‐L‐lysine (PSL) in various salt solutions were studied by use of ORD and potentiometric titration measurements. The addition of alkali metal salts to the fully ionized PGL or PSL solution cause