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Colorimetric determination of agarose-immobilized proteins by formation of copper-protein complexes

✍ Scribed by Dante J. Marciani; Scott D. Wilkie; Cheryl L. Schwartz

Book ID
107711865
Publisher
Elsevier Science
Year
1983
Tongue
English
Weight
653 KB
Volume
128
Category
Article
ISSN
0003-2697

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✍ R.Bryan Yamasaki; Dorothy A. Shimer; Robert E. Feeney πŸ“‚ Article πŸ“… 1981 πŸ› Elsevier Science 🌐 English βš– 622 KB

A new method is presented for the calorimetric determination of arginine residues in proteins. Under mildly alkaline conditions, p-nitrophenylglyoxal reacted with arginine to produce a stable colored solution in the presence of 0.15 M sodium ascorbate. Complete color development was obtained after 3