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Colorimetric assay of sialic acid by a methyl-3-benzothiazolinone-2-hydrazone reactant

โœ Scribed by Y. Massamiri; M. Beljean; G. Durand; J. Feger; M. Pays; J. Agneray

Book ID
102626158
Publisher
Elsevier Science
Year
1978
Tongue
English
Weight
418 KB
Volume
91
Category
Article
ISSN
0003-2697

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โœฆ Synopsis

A colorimetric assay of sialic acid has been developed in which bound sialic acid is oxidized by periodic acid so as to quantitatively release formaldehyde. In the second step of the reaction formaldehyde reacts with methyl-3-benzothiazolinone-2-hydrazone, giving a colored compound. This method does not require a prior hydrolysis and presents the advantage of great accuracy.

๐Ÿ“œ SIMILAR VOLUMES

Determination of Manganese Peroxidase Ac
Determination of Manganese Peroxidase Activity with 3-Methyl-2-benzothiazolinone Hydrazone and 3-(Dimethylamino)benzoic Acid
โœ M.D. Castillo; J. Stenstrom; P. Ander ๐Ÿ“‚ Article ๐Ÿ“… 1994 ๐Ÿ› Elsevier Science ๐ŸŒ English โš– 479 KB

This method was proposed earlier for measuring glucose in a peroxidase-glucose oxidase system but has not been studied for determination of manganese peroxidase (MnP) activity. The assay is based on the oxidative coupling of 3-methyl-2-benzothiazolinone hydrazone (MBTH) and 3-(dimethylamino)benzoic