Colony stimulating factor-1 stimulates diacylglycerol generation in murine bone marrow-derived macrophages, but not in resident peritoneal macrophages

✍ Scribed by Nurin Veis; John A. Hamilton

• Publisher: John Wiley and Sons
• Year: 1991
• Tongue: English
• Weight: 923 KB
• Volume: 147
• Category: Article
• ISSN: 0021-9541
• DOI: 10.1002/jcp.1041470215

✦ Synopsis

farkvdlr 3050, Australia Colony stimulating factor-1 (CSF-1) stimulates DNA synthesis in murine bone marrow-derived macrophages (BMM); however, unlike BMM, murine resident peritoneal macrophages (RPM) undergo a poor proliferative response. It has previously been shown that phosphatidylinositol-4,5-bisphosphate hydrolysis is not associated with CSF-1 action in BMM. In this report we demonstrate that, despite a lack of inositol trisphosphate generation, CSF-1 transiently elevated both [3Hlmyristoyl-and [3Hlarachidonyl-diacylglycerol (DAG) in BMM in a dosedependent fashion. CSF-1 failed, however, to stimulate an increase in either species of DAC in RPM. Thus, DAG could be a second messenger for the proliferative action of CSF-1 in rnacrophages. Other mitogenic agents, ? 2-O-tetradecanoyl phorbol 13-acetate (TPA) and exogenous phospholipase C, also increased BMM levels of [3H]myristoyl-and [3H]arachidonyl-DAG. The nonmitogenic agents, lipopolysaccharide (LPS), tumor necrosis factor-a (TNF-a) and rymosan, had different effects on the generation of either speciesof DAC in BMM.

LPS failed to elevate either form, TNF-(U increased only [-'HIarachidonyl-DAG, while zymosan stimulated levels ot' both species of DAG. It therefore appears that increased diacylglycerol generation may be necessary, but perhaps not sufficient, for macrophage proliferation.