Collagenolytic activity of a soil actinomycete
β Scribed by R. Chakraborty; A. L. Chandra
- Book ID
- 112923466
- Publisher
- Springer Netherlands
- Year
- 1982
- Tongue
- English
- Weight
- 251 KB
- Volume
- 27
- Category
- Article
- ISSN
- 0015-5632
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## Abstract The cellulolytic activity of several aerobic soil actinomycetes against insoluble cellulose and soluble cellulose derivatives (CMCβcarboxymethylcellulose) was studied. From the soil, 8 actinomycete strains were isolated after enrichment growth and purification on the same synthetic med
A new assay procedure for the determination of collagenolytic activity is presented. The substrate can be prepared by simple reduction of the purified acidsoluble rat tail tendon collagen with NaB3H4. Collagenase activity is determined by measurement of soluble tritiated collagen peptides released.