Collagen scaffolds loaded with collagen-binding NGF-β accelerate ulcer healing

✍ Scribed by Wenjie Sun; Hang Lin; Bing Chen; Wenxue Zhao; Yannan Zhao; Zhifeng Xiao; Jianwu Dai

• Publisher: John Wiley and Sons
• Year: 2009
• Tongue: English
• Weight: 320 KB
• Volume: 9999A
• Category: Article
• ISSN: 1549-3296
• DOI: 10.1002/jbm.a.32445

✦ Synopsis

Abstract

Studies have shown that exogenous nerve growth factor (NGF) accelerates ulcer healing, but the inefficient growth factor delivery system limits its clinical application. In this report, we found that the native human NGF‐β fused with a collagen‐binding domain (CBD) could form a collagen‐based NGF targeting delivery system, and the CBD‐fused NGF‐β could bind to collagen membranes efficiently. Using the rabbit dermal ischemic ulcer model, we have found that this targeting delivery system maintains a higher concentration and stronger bioactivity of NGF‐β on the collagen membranes by promoting peripheral nerve growth. Furthermore, it enhances the rate of ulcer healing through accelerating the re‐epithelialization of dermal ulcer wounds and the formation of capillary lumens within the newly formed tissue area. Thus, collagen membranes loaded with collagen‐targeting human NGF‐β accelerate ulcer healing efficiently. © 2009 Wiley Periodicals, Inc. J Biomed Mater Res, 2010