Rhodamine (rh) phalloidin was used as a probe for actin filaments in living eggs and was observed using confocal microscopy. Exogenous, rh phalloidin labeled actin filaments were injected into eggs and were observed to translocate in the cytoplasm. At fertilization, filaments accumulated at the fert
Cold shock induces actin reorganization and polyspermy in sea urchin eggs
โ Scribed by Santella, Luigia ;Monroy, Alberto
- Publisher
- John Wiley and Sons
- Year
- 1989
- Tongue
- English
- Weight
- 748 KB
- Volume
- 252
- Category
- Article
- ISSN
- 0022-104X
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โฆ Synopsis
The effect of low temperature on the cortical actin system and polyspermy in sea urchin eggs was studied. Eggs cold-shocked at 3ยฐC for 1 hour formed a cortical system of polymerized actin and were polyspermic when fertilized. These effects were completely reversible following a 7-20 minute recovery period at room temperature. The present data raises the possibility that actin, or components of the egg cytoskeleton, regulate sperm entry in sea urchin eggs.
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Elongation of microvilli and formation of actin filaments after treatment with a phorbol ester, TPA, were investigated in unfertilized eggs of Hemicentrotus pulcherrimus. Microvilli on the egg surface were examined by scanning electron microscopy. Actin filaments in the cortical layer of the eggs we
Changes in the intracellular Ca2+ concentration ([Ca2+]i) and the formation of actin filaments were investigated in unfertilized eggs of the sea urchin Hemicentrotus pulcherrimus after activation with a phorbol ester, 12-O-tetradecanoyl phorbol13-acetate (TPA). Intracellular Ca2+ oscillation was obs