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Coincidence of Cleavage Sites of Intron Endonuclease I-TevI and Critical Sequences of the Host Thymidylate Synthase Gene

✍ Scribed by David R. Edgell; Matthew J. Stanger; Marlene Belfort


Book ID
116661650
Publisher
Elsevier Science
Year
2004
Tongue
English
Weight
678 KB
Volume
343
Category
Article
ISSN
0022-2836

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The chloroplast ribosomal intron of Chlamydomonas reinhardtii encodes a sequence-specific DNA endonuclease (I-CreI), which is most probably involved in the mobility of this intron. Here we show that I-CreI generates a 4 bp staggered cleavage just downstream of the intron insertion site. The I-CreI r