Characterization of the cleavage site and the recognition sequence of the I-CreI DNA endonuclease encoded by the chloroplast ribosomal intron of Chlamydomonns reinhardtii

✍ Scribed by Dürrenberger, Franz ;Rochaix, Jean-David

• Book ID: 104699623
• Publisher: Springer
• Year: 1993
• Tongue: English
• Weight: 874 KB
• Volume: 236-236
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00277141

✦ Synopsis

The chloroplast ribosomal intron of Chlamydomonas reinhardtii encodes a sequence-specific DNA endonuclease (I-CreI), which is most probably involved in the mobility of this intron. Here we show that I-CreI generates a 4 bp staggered cleavage just downstream of the intron insertion site. The I-CreI recognition sequence is 19-24 bp in size and is located asymmetrically around the intron insertion site. Screening of natural variants of the I-CreI recognition sequence indicates that the I-CreI endonuclease tolerates single and even multiple base changes within its recognition sequence.