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Co-expression of heparin-binding EGF-like growth factor and related peptides in human gastric carcinoma

✍ Scribed by Markus Naef; Munehiro Yokoyama; Helmut Friess; Markus W. Büchler; Murray Korc

Publisher
John Wiley and Sons
Year
1996
Tongue
French
Weight
898 KB
Volume
66
Category
Article
ISSN
0020-7136

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✦ Synopsis

Heparin-binding epidermal growth factor (EGF)-like growth factor (HB-EGF) is a member of the EGF family of polypeptide growth factors, which includes EGF, transforming growth faaor a (TGF-a), amphiregulin (AR) and betacellulin (BTC). To assess the potential role of HB-EGF in human gastric carcinomas, the expression of HB-EGF and EGF receptor (EGF-R) was examined in normal and cancerous gastric tissues and cultured gastric cancer cell lines. By Northern blot analysis, there was a 4.7-fold increase in HB-EGF mRNA levels in human gastric cancers compared with normal gastric tissues. There was a concomitant 3.9-fold increase in EGF-R mRNA levels in these cancers. lmmunostaining revealed co-localization in 72% of the cancer cells of HB-EGF and EGF-R AR and BTC moieties were not evident by Northern blot analysis. However, using PCR, both AR and BTC mRNA species were demonstrated in normal and cancerous gastric tissues. By Northem blot analysis, HB-EGF, TGF-a. AR, BTC and EGF-R mRNA moieties were co-expressad in KATO 111 and NCLN87 gastric cancer cell lines. Furthermore, HB-EGF, EGF and TGF-a enhanced the growth of both cell lines in a dose-dependent manner. Our findings suggest that HB-EGF is relatively abundant in human gastric cancers and that coexpression of the EGF ligand family may lead to excessive activation of EGF-R in this disorder.

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