## Abstract Calcium‐phosphate/deoxyribose nucleic acid (Ca‐P/DNA) co‐precipitates were deposited on or encapsulated in fast‐degrading polymer films with surface erosion degradation mechanism to mediate cell transfection. The polymer, cholic acid functionalized star poly(DL‐lactide), was synthesized
Co-electrospun fibrous scaffold–adsorbed DNA for substrate-mediated gene delivery
✍ Scribed by Jun Zhang; Yajun Duan; Di Wei; Lianyong Wang; Hongjun Wang; Zhongwei Gu; Deling Kong
- Book ID
- 102873705
- Publisher
- John Wiley and Sons
- Year
- 2010
- Tongue
- English
- Weight
- 631 KB
- Volume
- 96A
- Category
- Article
- ISSN
- 1549-3296
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## Background To overcome the extracellular barriers in gene delivery and direct gene delivery to target tissues, substrate-mediated transfection, which sustains the release of naked DNA or vector/DNA complexes, and also supports cell growth, has been developed. ## Methods In the present study,
## Abstract The method of coating electrospun ultrafine poly(L‐lactic acid) fibers with DNA, by building up polyelectrolyte layer(s) of poly(ethyleneimine) (PEI) and plasmid DNA using an electrostatic layer‐by‐layer deposition method, for gene delivery is presented. The pGL3 encoding luciferase was