Dendrimer/DNA complexes encapsulated functional biodegradable polymer for substrate-mediated gene delivery

✍ Scribed by Hui-Li Fu; Si-Xue Cheng; Xian-Zheng Zhang; Ren-Xi Zhuo

• Book ID: 102891008
• Publisher: John Wiley and Sons
• Year: 2008
• Tongue: English
• Weight: 513 KB
• Volume: 10
• Category: Article
• ISSN: 1099-498X
• DOI: 10.1002/jgm.1258

✦ Synopsis

Background

To overcome the extracellular barriers in gene delivery and direct gene delivery to target tissues, substrate-mediated transfection, which sustains the release of naked DNA or vector/DNA complexes, and also supports cell growth, has been developed.

Methods

In the present study, polyamidoamine (PAMAM) dendrimer/DNA complexes encapsulated functional biodegradable polymer films for substratemediated gene delivery were prepared. To maintain the activity of DNA during dehydration, the dendrimer/DNA complexes were encapsulated in a water soluble polymer, poly α,β-[N-(2-hydroxyethyl)-L -aspartamide], and then deposited on or sandwiched in functional polymer films with a fast degradation rate to mediate gene transfection. The in vitro gene transfections of pGL3-Luc and pEGFP-C1 plasmids in HEK293 cells mediated by different films were studied. For comparison, the transfection mediated by the film fabricated by conventional linear poly( DL -lactide) was also investigated.

Results

The expression of pGL3-Luc and pEGFP-C1 plasmids could effectively be mediated by the PAMAM/DNA complexes deposited or sandwiched polymer films, with transfection efficiencies comparable to that of solution-based transfections. The cells on the functionalized star poly( DLlactide) film exhibited much higher gene expression compared to the cells on the conventional linear poly( DL -lactide) film because the fast degradation rate of star poly( DL -lactide) facilitated the access of PAMAM/DNA complexes for the cells seeded on the film. In addition, the films did not exhibit any additional cytotoxicity to the cells during the degradation and transfection.