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Co-amplification of c-myc/pvt-1 in immortalized mouse b-lymphocytic cell lines results in a novel pvt-1/aj-l transcript

✍ Scribed by Konrad Huppi; David Siwarski; John D. Shaughnessy Jr.; J. Frederic Mushinski

Publisher: John Wiley and Sons
Year: 1993
Tongue: French
Weight: 709 KB
Volume: 53
Category: Article
ISSN: 0020-7136
DOI: 10.1002/ijc.2910530323

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✦ Synopsis

In a series of mouse pvt-I cDNA clones prepared from an immortalized B-cell line that contains an amplified c-rnyclpvt-I region, we have identified a unique cDNA, AJ-I, which contains 57 bp of pvt-I sequence (pvt-la) spliced to a novel sequence of 2.1 kb. We report here that this 3 ' segment (termed Aj-IX) maps more than 60 kb telomeric to pvt-la and is encoded by a novel locus (A]-I) on mouse chromosome 15. The Aj-IX probe detects a transcript that is expressed only in normal mouse brain and testis. Several mast-cell tumors, Ly-I + B-lymphocytic cell lines and a neuroblastoma also display abundant levels of Aj-IX-specific mRNA. However, splicing of pvt-la to Aj-IX is found exclusively in Ly-I + B-cell lines that contain amplified c-rnyclpvt-I. We conclude that some feature in the generation of this amplicon facilitates the synthesis of a pvt-IlAj-IX chimeric mRNA that may play a role in immortalization of these Ly-I + B-cell lines.

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