Chimeric transcripts with an open reading frame are generated as a result of translocation to the Pvt-1 region in mouse B-cell tumors
✍ Scribed by Konrad Huppi; David Siwarski
- Publisher
- John Wiley and Sons
- Year
- 1994
- Tongue
- French
- Weight
- 515 KB
- Volume
- 59
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Some mouse plasmacytomas exhibit a t(6; 15) chromosomal translocation in which the breakpoint resides within the Pvt-I locus located 260 kilobases (kb) downstream of c-myc on mouse chromosome 15. In this report, we show that the Pvt-I locus does not exhibit allelic exclusion in that Pvt-I transcripts continue to be expressed from the non-translocated allele in t(6; 15) plasmacytomas. From the translocated allele, we find chimeric transcripts containing a short 57-bp segment of Pvt-I (termed Pvt-I a ) spliced directly to the immunoglobulin constant region sequence (Ig-Ck). These short transcripts have replaced a Jk segment with a trytophan residue via RNA splicing and contain a continuous open reading frame (ORF) from Pvtl a through Ck. Since this Pvt-IalCk transcript is found in all 3 t(6; 15) plasmacytomas examined, regardless of the location of the chromosomal breakpoint, we suggest that the Pvt-IalCk chimera may have a functional role in the development of mouse plasmacytomas. G 1994 Wiley-Liss, lnc. * Chromosomal translocations that juxtapose f i t -1 or c-myc to one of the immunoglobulin (Ig) light-or heavy-chain gene loci