Clostridium difficile: Methods and Protocols (Methods in Molecular Biology, 646)

✍ Scribed by Peter Mullany (editor), Adam P. Roberts (editor)

Publisher: Humana
Year: 2010
Tongue: English
Leaves: 235
Category: Library

No coin nor oath required. For personal study only.

✦ Synopsis

Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide. In Clostridium difficile: Methods and Protocols, expert researchers bring together the most recently developed methods for studying the organism, including techniques involving isolation, molecular typing, genomics, genetic manipulation, and the use of animal models. Written in the highly successful Methods in Molecular Biology™ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.

Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols serves as an ideal guide for scientists now in a position to gain an in-depth understanding of how this organism is transmitted and how it causes disease.

✦ Table of Contents

Clostridium difficile
Preface
Contents
Contributors
Part I Introduction to Clostridium difficile and the Disease
It Causes
Part II Isolation and Culture Techniques
Part III Methods for Typing and Epidemiological Studies
Part IV Biochemistry of the Organism
Part V Genomics
Part VI Development of Systems for Genetic Analysis of the Organism
Part VII Animal Models of Disease
SUBJECT INDEX

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