<p>This second edition provides 21 new chapters on methods used in laboratories for investigating the physiology and molecular genetics of the pathogen <i>Clostridium difficile</i>. Chapters detail up-to -date experimental techniques for gene editing and transcriptional analysis which are used to in
Clostridium difficile: Methods and Protocols
β Scribed by Adam P. Roberts, Peter Mullany (auth.), Peter Mullany, Adam P. Roberts (eds.)
- Publisher
- Humana Press
- Year
- 2010
- Tongue
- English
- Leaves
- 237
- Series
- Methods in Molecular Biologyβ’ 646
- Edition
- 1
- Category
- Library
No coin nor oath required. For personal study only.
β¦ Synopsis
Clostridium difficile, a major nosocomial pathogen shown to be a primary cause of antibiotic-associated disease, has emerged as a highly transmissible and frequently antibiotic-resistant organism, causing a considerable burden on health care systems worldwide. In Clostridium difficile: Methods and Protocols, expert researchers bring together the most recently developed methods for studying the organism, including techniques involving isolation, molecular typing, genomics, genetic manipulation, and the use of animal models. Written in the highly successful Methods in Molecular Biologyβ’ series format, chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes highlighting tips on troubleshooting and avoiding known pitfalls.
Authoritative and cutting-edge, Clostridium difficile: Methods and Protocols serves as an ideal guide for scientists now in a position to gain an in-depth understanding of how this organism is transmitted and how it causes disease.
β¦ Table of Contents
Front Matter....Pages i-x
Front Matter....Pages 1-1
Front Matter....Pages 3-7
Front Matter....Pages 9-35
Front Matter....Pages 37-37
Front Matter....Pages 39-52
Front Matter....Pages 53-53
Front Matter....Pages 55-65
Back Matter....Pages 67-76
....Pages 77-90
β¦ Subjects
Microbiology; Pathology; Laboratory Medicine
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