An analytic formalism developed earlier to describe the time evolution of the basic enzyme reaction is extended to fully competitive systems. Time-dependent closed form solutions are derived for the three nominal cases of competition: even, slow and fast inhibitors, allowing for the first time the c
Closed Form Solution for Time-dependent Enzyme Kinetics
โ Scribed by S. Schnell; C. Mendoza
- Publisher
- Elsevier Science
- Year
- 1997
- Tongue
- English
- Weight
- 189 KB
- Volume
- 187
- Category
- Article
- ISSN
- 0022-5193
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โฆ Synopsis
Based on the quasi-steady-state approximation, a closed form solution for the total time evolution of the reactant concentrations in the basic enzyme-substrate reaction is reported for the first time. Such a solution is given in terms of the omega function, which satisfies the transcendental equation W(x)exp(W(x)) = x, and enables the generation of the corresponding time derivatives thus fully characterizing the system. The agreement with results obtained from both the inner (fast region) and outer (slow region) solutions of singular perturbation procedures is very good, but an advantage of the present formalism is the analytic representation of the transition where the perturbation methods are shown to be inaccurate.
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