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Closed circular DNA associated with yeast mitochondria

โœ Scribed by L. Zeman; C.V. Lusena

Book ID
115904223
Publisher
Elsevier Science
Year
1974
Tongue
English
Weight
249 KB
Volume
38
Category
Article
ISSN
0014-5793

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The alkaline preparation of prokaryotic plasmids (Birnboim and Doly, 1979) has been here adapted to yeast. By simple denaturation and renaturation steps we recovered, from Saccharomyces cerevisiae and Schizosaccharomyces pombe, a population of nucleic acid molecules highly enriched in circular forms

2 ฮผm Covalently closed non-mitochondrial
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A population of small covalently closed non-mitochondrial circular DNA molecules was isolated from the petite-negative yeast Schizosaccharomyces pombe. The mean length of these molecules, possessing the same density as nuclear DNA (1.695 g/cm3) is 1.95 +/- 0.18 micrometer. The presence of these mini