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Clonogenic in vitro growth and histologic grading of primary human breast tumors

โœ Scribed by Verena Hug; Rashid Rashid; George Blumenschein; Gary Spitzer


Book ID
112212002
Publisher
AlphaMed Press
Year
1985
Tongue
English
Weight
346 KB
Volume
3
Category
Article
ISSN
1066-5099

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We report a double-agar clonogenic system adapted to human breast cancer. We optimized the conditions for cell growth and clonogenicity with respect to hormones (insulin, estradiol, progesterone) and components of the extracellular matrix (collagen, laminin and fibronectin). Using our experimental i