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Cloning the Human Major Histocompatibility Complex in YACs

✍ Scribed by Hadi Abderrahim; Jean-Luc Sambucy; Francois Iris; Pierre Ougen; Alain Billault; Ilya M. Chumakov; Jean Dausset; Daniel Cohen; Denis Le Paslier


Book ID
115612387
Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
495 KB
Volume
23
Category
Article
ISSN
0888-7543

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Major histocompatibility complex restric
✍ Christian Schmitt; Michel CognΓ©; Monique Agrapart; Jean-Jacques Ballet πŸ“‚ Article πŸ“… 1984 πŸ› John Wiley and Sons 🌐 English βš– 658 KB

Peripheral blood mononuclear cells (PBMC) from an HLA DRw6/7 individual were stimulated with tetanus toxoid (TT). T cell blasts were cloned by the limiting dilution technique in the presence of TT and irradiated autologous PBMC (iPBMC). Twelve were propagated under interleukin 2 and restimulated wee