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Cloning the cyd gene locus coding for the cytochrome d complex of Escherichia coli

โœ Scribed by Green, George N.; Kranz, Janice E.; Gennisa, Robert B.

Book ID
120527371
Publisher
Elsevier Science
Year
1984
Tongue
English
Weight
828 KB
Volume
32
Category
Article
ISSN
0378-1119

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Cloning of cybB, the gene for cytochrome
Cloning of cybB, the gene for cytochrome b561 of Escherichia coli K12
โœ Murakami, Hiroshi ;Kita, Kiyoshi ;Anraku, Yasuhiro ๐Ÿ“‚ Article ๐Ÿ“… 1984 ๐Ÿ› Springer ๐ŸŒ English โš– 584 KB

A 37 kb fragment of DNA from an F-prime factor, F100-12, which showed a gene dosage effect on b-type cytochromes, was cloned with a cosmid vector, pHC79. Gel filtration of cytochromes and product analysis of the hybrid plasmids indicated that this fragment contained cybB, the structural gene for cyt