The genes for the ribosomal proteins $9 (rpsI) and L13 (rplM) of Escherichia coli have been cloned into a 2 phage vector termed L47.1. The two genes were identified by infecting UV-light irradiated cells with the resultant phages and analyzing the protein products by two-dimensional gel electrophore
โฆ LIBER โฆ
Cloning, sequencing and transcriptional analysis of a Streptomyces coelicolor operon containing the rplM and rpsI genes encoding ribosomal proteins ScoL13 and ScoS9
โ Scribed by Sanchez, C. ;Blanco, G. ;Mendez, C. ;Salas, J. A.
- Publisher
- Springer
- Year
- 1997
- Tongue
- English
- Weight
- 345 KB
- Volume
- 257
- Category
- Article
- ISSN
- 0026-8925
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A 3 kb genomic fragment containing the nusG gene of Streptomyces coelicolor A3(2) was identified, cloned and sequenced. Sequence analysis revealed 3 complete and 2 truncated open reading frames (ORFs): truncated ORFu (similar to a Bacillus gene encoding a thermostable aspartate aminotransferase)-sec