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Cloning, sequence analysis and heterologous expression of the Myrothecium gramineum orotidine-5′-monophosphate decarboxylase gene

✍ Scribed by Sofie L. De Maeseneire; Manu R.M. De Groeve; Thierry Dauvrin; Marjan De Mey; Wim Soetaert; Erick J.M.C. Vandamme


Book ID
109329427
Publisher
John Wiley and Sons
Year
2006
Tongue
English
Weight
763 KB
Volume
261
Category
Article
ISSN
0378-1097

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The URA3 gene of Candida utilis encoding orotidine-5'-phosphate decarboxylase enzyme was isolated by complementation in Escherichia coli pyrF mutation. The deduced amino-acid sequence is highly similar to that of the Ura3 proteins from other yeast and fungal species. An extensive analysis of the fam