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Cloning, overexpression and mechanistic studies of carboxyphosphonoenolpyruvate mutase from Streptomyces hygroscopicus

✍ Scribed by Scott J. POLLACK; Sally FREEMAN; David L. POMPLIANO; Jeremy R. KNOWLES

Book ID
115129643
Publisher
John Wiley and Sons
Year
1992
Tongue
English
Weight
940 KB
Volume
209
Category
Article
ISSN
1432-1327

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Nucleotide sequence of a carboxyphosphon
Nucleotide sequence of a carboxyphosphonoenolpyruvate phosphonomutase gene isolated from a bialaphos-producing organism, Streptomyces hygroscopicus, and its expression in Streptomyces lividans
✍ Hidaka, Tomomi ;Hidaka, Makoto ;Uozumi, Takeshi ;Seto, Haruo πŸ“‚ Article πŸ“… 1992 πŸ› Springer 🌐 English βš– 320 KB

The carboxyphosphonoenolpyruvate (CPEP) phosphonomutase gene of bialaphos-producing Streptomyces hygroscopicus, which encodes a C-P bond forming enzyme was cloned into Streptomyces lividans and sequenced. The amino acid composition of the protein coded in an open reading frame of 295 codons and its