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Cloning of theBacillus pumilusβ-xylosidase gene (xynB ) and its expression inSaccharomyces cerevisiae

✍ Scribed by D. C. La Grange; I. S. Pretorius; W. H. van Zyl

Publisher
Springer
Year
1997
Tongue
English
Weight
178 KB
Volume
47
Category
Article
ISSN
1432-0614

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Cloning and expression of theClostridium thermosulfurogenesD-xylose isomerase gene (xyLA) inSaccharomyces cerevisiae
✍ Catharine J. Moes; Isak S. Pretorius; Willem H. Zyl 📂 Article 📅 1996 🏛 Springer Netherlands 🌐 English ⚖ 503 KB

A recombinant strain of Saccharomyces cerevisiae containing the bacterial Dxylose isomerase (xylA) gene of Clostridium thermosulfirogenes was constructed. The .+A gene has high thermal stability, functions in an anaerobic environment and has a G+C content of 38.9%, which should render it a good cand