Cloning and expression of theClostridium thermosulfurogenesD-xylose isomerase gene (xyLA) inSaccharomyces cerevisiae

✍ Scribed by Catharine J. Moes; Isak S. Pretorius; Willem H. Zyl

• Publisher: Springer Netherlands
• Year: 1996
• Tongue: English
• Weight: 503 KB
• Volume: 18
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00142943

✦ Synopsis

A recombinant strain of Saccharomyces cerevisiae containing the bacterial Dxylose isomerase (xylA) gene of Clostridium thermosulfirogenes was constructed. The .+A gene has high thermal stability, functions in an anaerobic environment and has a G+C content of 38.9%, which should render it a good candidate for successful expression in S. cerevisiae. Efficient transcription of the XyrA gene under the control of the ADH2 gene promoter and terminator sequences in a recombinant S. cerevisiae strain was shown through Northern blot analysis. However, the xylA recombinant strain was unable to grow on D-X$OSTZ as sole carbon source.