Cloning of penicillin G acylase-encoding gene fromEscherichia colihigh-producing strain RE3

✍ Scribed by L. Sobotková; V. Štěpánek; K. Plháčková; P. Kyslík

• Book ID: 104633940
• Publisher: Springer Netherlands
• Year: 1995
• Tongue: English
• Weight: 430 KB
• Volume: 17
• Category: Article
• ISSN: 0141-5492
• DOI: 10.1007/bf00130358

✦ Synopsis

&a gene from the industrial strain of E. co/i RE3 hyperproducing penicillin G acylase (PGA) was cloned using a simple and rapid NIPAB-based chromogenic method for the detection of PGA-positive recombinant clones. Heterogeneous genetical material was prepared by subcloning pgu in vectors pACYC 184. pBR322-2 and pK19. The highest constitutive expression of pga was observed in a strain bearing the recombinant plasmid pKAl8 synthesizing 2.5 times more of enzyme than the parent strain.