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Cloning of penicillin acylase fromEscherichia coli: Catalytic properties of recombinant enzymes

✍ Scribed by A. S. Yasnaya; O. V. Yamskova; D. T. Guranda; T. A. Shcherbakova; V. I. Tishkov; V. K. Švedas


Book ID
111494696
Publisher
Allerton Press Inc
Year
2008
Tongue
English
Weight
185 KB
Volume
63
Category
Article
ISSN
0027-1314

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Cloning of penicillin G acylase-encoding
✍ L. Sobotková; V. Štěpánek; K. Plháčková; P. Kyslík 📂 Article 📅 1995 🏛 Springer Netherlands 🌐 English ⚖ 430 KB

&a gene from the industrial strain of E. co/i RE3 hyperproducing penicillin G acylase (PGA) was cloned using a simple and rapid NIPAB-based chromogenic method for the detection of PGA-positive recombinant clones. Heterogeneous genetical material was prepared by subcloning pgu in vectors pACYC 184. p