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Cloning of new acylamidase gene fromRhodococcus erythropolisand its expression inEscherichia coli

โœ Scribed by Lavrov, K. V.; Yanenko, A. S.

Book ID
121616258
Publisher
SP MAIK Nauka/Interperiodica
Year
2013
Tongue
English
Weight
446 KB
Volume
49
Category
Article
ISSN
1022-7954

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Molecular cloning of a new endoglucanase
Molecular cloning of a new endoglucanase gene fromClostridium cellulolyticumand its expression inEscherichia coli
โœ Seigo Shima; Yasuo Igarashi; Tohru Kodama ๐Ÿ“‚ Article ๐Ÿ“… 1991 ๐Ÿ› Springer ๐ŸŒ English โš– 481 KB

Two genes coding for endoglucanase activity in Clostridium cellulolyticum were cloned and expressed in Escherichia coli by using plasmid pUC18. The sizes of two fragments harbouring endoglucanase genes are 4.4 kb and 2.0 kb, respectively. The 2.0-kb fragment was identical with a reported D N A fragm