𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Cloning of murine CDK9/PITALRE and its tissue-specific expression in development

✍ Scribed by Luigi Bagella; Timothy K. MacLachlan; Russell J. Buono; M. Michele Pisano; Antonio Giordano; Antonio De Luca

Publisher
John Wiley and Sons
Year
1998
Tongue
English
Weight
762 KB
Volume
177
Category
Article
ISSN
0021-9541

No coin nor oath required. For personal study only.

✦ Synopsis

The cdc2-family of serine/threonine kinases and their binding partners recently were implicated in developmental roles. We previously cloned a cdc2-related kinase, cdk9/PITALRE, that is able to phosphorylate the retinoblastoma protein in vitro. We describe here the cloning and the characterization of the mouse homolog of cdk9/PITALRE. The murine cDNA is 98% identical with humans and is expressed at high levels in brain and kidney tissues. The kinase activity and protein expression of cdk9/PITALRE were highest in terminally differentiated tissues such as the muscle and brain. In situ immunohistology and immunofluorescence detected cdk9/PITALRE protein not only within terminally differentiated cells such as muscle and neuronal cells, but also in proliferating cells. C2C12 and P19 cells induced to differentiate along muscle and neural lineages peaked in cdk9/PITALRE kinase activity at the end of differentiation. These results suggest that, among other roles, cdk9/PITALRE plays a role not unlike cdk5 in the differentiation of certain cell types.

πŸ“œ SIMILAR VOLUMES

Molecular cloning and tissue-specific ex
Molecular cloning and tissue-specific expression of Mrad9, a murine orthologue of the Schizosaccharomyces pombe rad9+ checkpoint control gene
✍ Haiying Hang; Sarah J. Rauth; Kevin M. Hopkins; Scott K. Davey; Howard B. Lieber πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 253 KB πŸ‘ 1 views

We have isolated a murine cDNA, Mrad9, that is orthologous to the fission yeast rad9 / and human HRAD9 genes. Mrad9 encodes a 389 amino acid long, 42,032 Dalton protein that is 27%identical and 56% similar to Rad9p, and 82% identical and 88% similar to HRAD9, at the amino acid level. Expression of t

Tissue-specific expression of murine Nkx
Tissue-specific expression of murine Nkx3.1 in the male urogenital system
✍ Peter J. Sciavolino; Elliott W. Abrams; Lu Yang; Leif P. Austenberg; Michael M. πŸ“‚ Article πŸ“… 1997 πŸ› John Wiley and Sons 🌐 English βš– 478 KB

The molecular mechanisms involved in growth and morphogenesis of the mammalian urogenital system are largely undefined. In this study, we describe the cloning and characterization of a novel murine homeobox gene, Nkx3.1, which is expressed in the male urogenital system during late embryogenesis and

Tissue-specific expression of Riken 2810
Tissue-specific expression of Riken 2810430M08 and its function in T-cell immune responses
✍ Tao Wu; ShenJiang Hu; Xiaoying Qiao; Hongyu Luo; Jiangping Wu πŸ“‚ Article πŸ“… 2007 πŸ› John Wiley and Sons 🌐 English βš– 483 KB

## Abstract Riken 2810430M08 is a gene with unknown functions. According to in situ hybridization (ISH), it presented a pattern of temporal expression, peaking in the mid‐gestation (embryonic Days e9–e14) stage in most tissues. In the late‐gestation stage and during the adulthood, Riken 2810430M08

Region-specific expression of cyclin-dep
Region-specific expression of cyclin-dependent kinase 5 (cdk5) and its activators, p35 and p39, in the developing and adult rat central nervous system
✍ Zheng, Min ;Leung, Conrad L. ;Liem, Ronald K. H. πŸ“‚ Article πŸ“… 1998 πŸ› John Wiley and Sons 🌐 English βš– 944 KB

The ubiquitously expressed cyclinzone, which may restrict cdk5 activation to the postmidependent kinase 5 (cdk5) is essential for brain develtotic neural cells in the developing brain. The expresopment. Bioactivation of cdk5 in the brain requires sion levels of p35 and p39 mRNAs in the marginal the

Cloning of the human uroplakin 1B cDNA a
Cloning of the human uroplakin 1B cDNA and analysis of its expression in urothelial-tumor cell lines and bladder-carcinoma tissue
✍ Jennie L. Finch; John Miller; James O. Aspinall; Prudence A. Cowled πŸ“‚ Article πŸ“… 1999 πŸ› John Wiley and Sons 🌐 French βš– 108 KB πŸ‘ 2 views

The human uroplakin 1B (UPK1B) gene codes for a structural protein which is a terminal differentiation component of the asymmetric unit membrane on the apical surface of the mammalian bladder. UPK1B is a member of the tetraspan family of proteins, many of which have de-regulated patterns of expressi