Cloning of IL-2-induced human lymphokine-activated killer (LAK) cells and their clinical utilization
β Scribed by Ning Mao; Pei-Hsien Tang; Min-Wei Zhang; Fei-Zi Jiang; De-Lin Du
- Book ID
- 112212323
- Publisher
- AlphaMed Press
- Year
- 1991
- Tongue
- English
- Weight
- 66 KB
- Volume
- 9
- Category
- Article
- ISSN
- 1066-5099
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Highly purified lymphocytes (> 99%) and monocytes (>90%) were isolated by CCE from peripheral blood of healthy donors. Blood lymphocytes were separated by this CCE into 9 subpopulations. The NK activities of these lymphocyte fractions against NK-sensitive K-562 cells and their LAK activities against
Clones capable of lysing fresh, uncultured tumor cells ("lymphokine-activated killer": "LAK' activity) were selected from microcultures derived from either E-rosette-positive or Erosette-negative cell populations. All the selected clones displayed a strong cytolytic activity against the NK-sensitive