Phenotypic and functional analysis of human CD3 + and CD3− clones with “lymphokine-activated killer” (LAK) activity. Frequent occurrence of CD3 + LAK clones which produce interleukin-2
✍ Scribed by M. C. Mingari; S. Ferrini; D. Pende; C. Bottino; I. Prigione; A. Moretta; L. Moretta
- Publisher
- John Wiley and Sons
- Year
- 1987
- Tongue
- French
- Weight
- 458 KB
- Volume
- 40
- Category
- Article
- ISSN
- 0020-7136
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✦ Synopsis
Clones capable of lysing fresh, uncultured tumor cells ("lymphokine-activated killer": "LAK' activity) were selected from microcultures derived from either E-rosette-positive or Erosette-negative cell populations. All the selected clones displayed a strong cytolytic activity against the NK-sensitive K562 cell line. Two major phenotypic groups of clones could be identified: a first group expressed the CD3 differentiation antigen, present exclusively on mature T lymphocytes; however, in contrast to typical cytolytic T lymphocytes, the majority of these clones expressed the unusual CD4-CD8phenotype, whereas the remainder were CD4 -CD8 + . A second group was represented by CD3-clones which, in most instances, expressed the T-cell-lineage-specific CD2 antigen. Following stimulation with phytohemagglutinin (PHA), most of the CD3 + LAK clones produced Interleukin-2 (IL-2) and interferon-? (IFN-y) whereas those expressing the CD3phenotype did not. Since previous studies indicated that PHA may be inefficient in inducing lymphokine production by Tcell variants lacking the CD3/T cell receptor complex (TCR), CD3clones were further stimulated with the calcium ionophore A23187 plus phorbol 12-myristate 13-acetate (PMA). Only 2/1 I CD3 -LAK clones produced small amounts of IL-2, whereas the majority released IFN-y. Given the peculiar phenotypic and functional properties of many CD3 + LAK clones, we suggest that they may belong to a T-cell subset distinct from typical CTLs. Exp. releasespont. release Max. releasespont. release Specific lysis = x 100.