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Cloning of an EcoRI fragment carrying E. coli tufA gene

โœ Scribed by Shibuya, Masabumi ;Nashimoto, Hiroko ;Kaziro, Yoshito

Publisher
Springer
Year
1979
Tongue
English
Weight
427 KB
Volume
170
Category
Article
ISSN
0026-8925

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โœฆ Synopsis

EcoRI fragments of the transducing phage lambda fus3 DNA have been linked to the ColEl derivative plasmid RSF2124 (ColEl-Apr) DNA using bacteriophage T4 ligase. Among the plasmids formed, one designated pTUAl was found to contain the E. coli tufA gene. The proof for the presence of tufA gene in pTUAl is based on the following observations: (1) ability of pTUAl DNA and is EcoRI fragments to direct synthesis of EF-Tu in a cell-free protein synthesizing system; and (2) RNA . DNA hybridization of RNA transcribed from phage lambda rifd18 carrying tufB with DNA from pTUAl.

๐Ÿ“œ SIMILAR VOLUMES

Cloning of E. coli pnp gene from an epis
Cloning of E. coli pnp gene from an episome
โœ Portier, Claude ;Migot, Caroline ;Grunberg-Manago, Marianne ๐Ÿ“‚ Article ๐Ÿ“… 1981 ๐Ÿ› Springer ๐ŸŒ English โš– 789 KB

Starting with an F' episome harboring a transposon inserted in the pnp gene (Portier 1980), we were able to identify an EcoRI restriction fragment carrying the pnp and argG genes. This fragment, from both wild-type and mutant episomes, was cloned ni pACYC184. The presence of argG on the fragment all