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Cloning of all EcoRI fragments from phage λ in E. coli

✍ Scribed by BERNARDI, ALBERTO; BERNARDI, GIORGIO


Book ID
109702870
Publisher
Nature Publishing Group
Year
1976
Tongue
English
Weight
379 KB
Volume
264
Category
Article
ISSN
0028-0836

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Cloning of an EcoRI fragment carrying E.
✍ Shibuya, Masabumi ;Nashimoto, Hiroko ;Kaziro, Yoshito 📂 Article 📅 1979 🏛 Springer 🌐 English ⚖ 427 KB

EcoRI fragments of the transducing phage lambda fus3 DNA have been linked to the ColEl derivative plasmid RSF2124 (ColEl-Apr) DNA using bacteriophage T4 ligase. Among the plasmids formed, one designated pTUAl was found to contain the E. coli tufA gene. The proof for the presence of tufA gene in pTUA